Photoprotective (UVB) Salen-Manganese Complex (EUK-134): Melbourne Dermatology Skin Care

Clinical Procedures and Topics

abbreviations accumulation activated activation against agent alleviate alyria amidinopropane associated before belgium catalase cell cells center companies complex complexes compounds concentration confer conferred consequently correspondence damage danielÊmaes davidÊdecraene davidÊgan department dependent dermatology dihydrochloride direct disease dismutase effect efficacy email exhibit exposed extracellular fashion following furthermore garmyn generates human hydrogen hypothesize induced inhibits investigated irradiation kapucijnenvoer katrienÊsmaers keratinocytes keywords kinase kinases kuleuven lauder leuven level lieveÊdeclercqà lotion lower lowers manganese mapk marjan marjanÊgarmyn maryÊmatsui melville membrane mimetic mitogen models noshade oevel online oxidative oxygen pathways peroxide pharmacologic phosphorylation photoprotective protection protein reduces regulated salen signal size stress superoxide terminal ultraviolet ÊfebruaryÊ

Photoprotective (UVB) Salen-Manganese Complex (EUK-134)

Salen-manganese complexes exhibit powerful superoxide dismutase and catalase activity, with pharmacologic efficacy in several oxidative-stress-associated disease models.

Ultraviolet UVB not only induces direct DNA damage, but also generates oxidative stress.

EUK-134, a salen-manganese complex, might therefore confer a direct protection against UVB-induced oxidative stress and consequently alleviate UVB-damage-induced signal transduction.

We investigated the effect of EUK-134 on the UVB-induced accumulation and stabilization of the p53 protein.

Cells treated with EUK-134 before UVB irradiation showed a significantly lower accumulation of the p53 protein in a concentration-dependent fashion.

Furthermore, EUK-134 severely reduced N-terminal phosphorylation of p53.

The extracellular signal-regulated kinase ERK and the stress-activated kinases JNK and p38 have been implicated in the UVB-induced N-terminal phosphorylation and accumulation of p53.

Pre-treatment with EUK-134 inhibited the UVB-induced activation of these mitogen-activated protein kinase (MAPK) pathways.

We hypothesize that EUK-134, by direct protection of the membrane from UVB-induced oxidative damage, reduces oxidative stress induced MAPK signaling and consequently lowers the level of p53 induction.

The protection conferred by EUK-134 resulted in a significant increase in cell survival following UVB irradiation.

A Synthetic Superoxide Dismutase/Catalase Mimetic (EUK-134) Inhibits Membrane-Damage-Induced Activation of Mitogen-Activated Protein Kinase Pathways and Reduces p53 Accumulation in Ultraviolet B-Exposed Primary Human Keratinocytes

David Decraene*, Katrien Smaers*, David Gan , Tom Mammone , Mary Matsui , Daniel Maes , Lieve Declercqà and Marjan Garmyn*

Department of Dermatology, University of Leuven, Leuven, Belgium

Estée Lauder Companies, Melville, New York, USA

àEstée Lauder Coordination Center, Oevel, Belgium

Correspondence: Marjan Garmyn, Department of Dermatology, UZ St. Rafaël, Kapucijnenvoer 33, B-3000 Leuven, Belgium. Email: Marjan.Garmyn@med.kuleuven.ac.be

Received 14 February 2003; Revised 4 August 2003; Accepted 22 October 2003; Published online 12 February 2004.

Keywords: oxidative stress, skin, p53 phosphorylation, manganese compounds, hydrogen peroxide.

Abbreviations: AAPH, 2,2'-azobis(2-amidinopropane)dihydrochloride; ERK, extracellular signal-regulated kinase; JNK/SAPK, c-Jun N-terminal kinase/stress-activated protein kinase; MAPK, mitogen-activated protein kinase; ROS, reactive oxygen species; SOD, superoxide dismutase; UV, ultraviolet.

Related Skin Care Information, Products and Expert Discussions

Home

Perennial / Wisdoms

Skin Care

Facial Skin Condition Treatments

Body Skin Condition Treatments

Skin Care Ingredients

Clinical Procedures and Topics

Photoprotective (UVB) Salen-Manganese Complex (EUK-134)


*72,000+ expert documents for the skincare aficionado.* Home View Cart/Checkout Contact/Customer Service Perennial / Wisdoms UV Exposure Aging Skin and Mature Skin Theories of Aging Antioxidants Sunscreens Skin Care Allergan Alyria Anthelios Avene Biomedic Blue Lizard Bull Frog Cellex-C Citrix Clarisonic Colorescience Cotz Clinique Medical DCL Dermatix Elastiderm Elta MD Estion Ethocyn Exuviance Fake Bake Frederic Fekkai Glycolix Glytone Great Lips Rx Heliocare IS Clinical Jane Iredale Jan Marini Kinerase Kinerase Clear Kinerase PhotoFacials Kinerase Pro+ Leaf & Rusher La Roche Posay L-M-X M Lab Neoceuticals Neocutis Neostrata Neova Nia24 Nordic Naturals Obagi Obagi Clenziderm Obagi Professional-C Obagi Rosaclear OC Eight Olos PCA Skin Pentaxyl Prevage MD Remergent Replenix RevaleSkin Revitalash Scarguard Skinceuticals SkinMedica Teamine Theraplex Ti-Silc / Z-Silc Topix Tricomin VitaMedica Zeno MD Browse more brands. Facial Skin Condition Treatments Adult Acne Broken Capillaries Dark Circles Deep Wrinkles Dry Skin Fine Lines Hyperpigmentation Open Pores Puffy Eyes Rosacea Sagging Skin Body Skin Condition Treatments Aging Hands Breast Firming Cellulite Hands Keratosis Pilaris Lips Scalp Acne Stretch Marks Skin Care Ingredients Alpha Lipoic Acid Arginine + Serine Asiatic Acid Blueberry Caffeine Capryloyl Salicylic Acid Ceramides Chlorogenic Acid Chrysin Coffee Berry Hydrolyzed Hyaluronic Acid Mexoryl Pro-Xylane Silymarin Sodium Laureth Sulfate/Sulphate Browse more ingredients. Clinical Procedures and Topics Four Fundamentals Absurd Skin Care Aging Skin Antioxidants Botox Enzyme Exfoliation Free Radical Damage French Skin Care Glycolic Acid Idebenone Irrational Fears Klein Becker Oxidative Stress Paula Begoun Perricone Restylane Skin Structure Stem Cells Healthy Skin Barrier Function Smoking Sun Protection Topical Vitamin C/Firming Choices and Needs Strivectin Additional clinical skincare topics. abbreviations accumulation activated activation against agent alleviate alyria amidinopropane associated before belgium catalase cell cells center companies complex complexes compounds concentration confer conferred consequently correspondence damage danielÊmaes davidÊdecraene davidÊgan department dependent dermatology dihydrochloride direct disease dismutase effect efficacy email exhibit exposed extracellular fashion following furthermore garmyn generates human hydrogen hypothesize induced inhibits investigated irradiation kapucijnenvoer katrienÊsmaers keratinocytes keywords kinase kinases kuleuven lauder leuven level lieveÊdeclercqà lotion lower lowers manganese mapk marjan marjanÊgarmyn maryÊmatsui melville membrane mimetic mitogen models noshade oevel online oxidative oxygen pathways peroxide pharmacologic phosphorylation photoprotective protection protein reduces regulated salen signal size stress superoxide terminal ultraviolet ÊfebruaryÊ
	Photoprotective (UVB) Salen-Manganese Complex (EUK-134) EUK-134 Salen-Manganese Complex is a UVB photoprotective agent, for supplementary use with sunscreens, available in Alyria Corrective Protection SPF 30 Lotion. Salen-manganese complexes exhibit powerful superoxide dismutase and catalase activity, with pharmacologic efficacy in several oxidative-stress-associated disease models. Ultraviolet UVB not only induces direct DNA damage, but also generates oxidative stress. EUK-134, a salen-manganese complex, might therefore confer a direct protection against UVB-induced oxidative stress and consequently alleviate UVB-damage-induced signal transduction. We investigated the effect of EUK-134 on the UVB-induced accumulation and stabilization of the p53 protein. Cells treated with EUK-134 before UVB irradiation showed a significantly lower accumulation of the p53 protein in a concentration-dependent fashion. Furthermore, EUK-134 severely reduced N-terminal phosphorylation of p53. The extracellular signal-regulated kinase ERK and the stress-activated kinases JNK and p38 have been implicated in the UVB-induced N-terminal phosphorylation and accumulation of p53. Pre-treatment with EUK-134 inhibited the UVB-induced activation of these mitogen-activated protein kinase (MAPK) pathways. We hypothesize that EUK-134, by direct protection of the membrane from UVB-induced oxidative damage, reduces oxidative stress induced MAPK signaling and consequently lowers the level of p53 induction. The protection conferred by EUK-134 resulted in a significant increase in cell survival following UVB irradiation. A Synthetic Superoxide Dismutase/Catalase Mimetic (EUK-134) Inhibits Membrane-Damage-Induced Activation of Mitogen-Activated Protein Kinase Pathways and Reduces p53 Accumulation in Ultraviolet B-Exposed Primary Human Keratinocytes David Decraene, Katrien Smaers, David Gan , Tom Mammone , Mary Matsui , Daniel Maes , Lieve Declercqà and Marjan Garmyn* Department of Dermatology, University of Leuven, Leuven, Belgium Estée Lauder Companies, Melville, New York, USA àEstée Lauder Coordination Center, Oevel, Belgium Correspondence: Marjan Garmyn, Department of Dermatology, UZ St. Rafaël, Kapucijnenvoer 33, B-3000 Leuven, Belgium. Email: Marjan.Garmyn@med.kuleuven.ac.be Received 14 February 2003; Revised 4 August 2003; Accepted 22 October 2003; Published online 12 February 2004. Keywords: oxidative stress, skin, p53 phosphorylation, manganese compounds, hydrogen peroxide. Abbreviations: AAPH, 2,2'-azobis(2-amidinopropane)dihydrochloride; ERK, extracellular signal-regulated kinase; JNK/SAPK, c-Jun N-terminal kinase/stress-activated protein kinase; MAPK, mitogen-activated protein kinase; ROS, reactive oxygen species; SOD, superoxide dismutase; UV, ultraviolet. Related Skin Care Information, Products and Expert Discussions Green Tea Polyphenols Prevent Ultraviolet Light-Induced Oxidative Damage and Matrix Metalloproteinases Expression in Mouse Skin Green tea polyphenol (—)-epigallocatechin-3-gallate treatment of human skin inhibits ultraviolet radiation-induced oxidative stress Flavonolignans from Silybum Marianum Moderate UVA-Induced Oxidative Damage to HaCaT Keratinocytes Cosmetic Dermatology Newsletter Summer 2007 New Roles of Low Density Lipoproteins and Vitamin E in the Pathogenesis of Atherosclerosis Alyria : Alyria Corrective Protection SPF 30 Oil Free Lotion : Photoprotective (UVB) Salen-Manganese Complex (EUK-134) : Alyria Corrective Protection SPF 30 Oil Free Lotion Sunscreen Ingredients : Alyria Corrective Protection SPF 30 Oil Free Lotion Ingredients : File Photoprotective (UVB) Salen-Manganese Complex (EUK-134) for reference.	August 2010 Special Preparation / Special Order Items July 2010 SkinMedica Chemical Peels Nia24 Reference Manual June 2010 New Anthelios 45 Sunscreens for Face and Body Replenix Anti-Photoaging Sunscreen SPF 45 VitaMedica HA + A Program May 2010 New Skinceuticals Sheer Physical UV Defense SPF 50 Alyria Antioxidant Capsules (Vitamin C + E) IS Clinical Youth Complex April 2010 RevaleSkin — CoffeeBerry Extract Retin-A Gel Availability March 2010 RevaleSkin Day Cream SPF 15 Trehalose February 2010 Injuv Polyphenols Kinerase Pro+ C8 Peptide Intensive Treatment with Kinetin and Zeatin January 2010 La Roche-Posay Redermic December 2009 Anti-Aging Sunscreen Reminders November 2009 Complimentary Shipping The Sun Zinc: An Important Antioxidant for Skin La Roche-Posay Derm AOX Biomedic Potent-C 10.5 Concentrate Tocotrienols Skinceuticals Pigment Regulator SkinMedica Retinol Complex October 2009 The Connection Between Oxidative Stress and Aging Definition of Oxidative Stress Sources of Free Radical Damage Ozone Obagi Professional C Ingredients Citrix Antioxidant Body Lotion September 2009 Tricomin Erythema from Retinoids Carnosine Interleukin-6 (IL-6) Asiaticoside vs. Madecassoside for Collagen Synthesis New Elta MD UV Clear SPF 46 New DCL Super Sheer Sunscreen SPF 50+ PCA pHaze 16+ C-Strength 20% Arginine and Serine Chrysin August 2009 Glytone Lipo-Lift Serum Ergothioneine Ubiquinone (Co-Enzyme Q-10) Tetrahexyldecyl Ascorbate Hydrolyzed Hyaluronic Acid Polymorphous Light Eruption Saccharomyces Ferment (Filtrate Lysate) La Roche-Posay Effaclar AI Intensive Acne Spot Treatment New Skinceuticals Retexturing Activator New Vivite Defining Lip Plumper Neova Advanced Essential Lash July 2009 Ceramide 3 Oxido Reductases Cellex-C vs. Skinceuticals D-Tocopheryl Glucoside Human Fibroblast Conditioned Media Kinerase Pro+ Therapy Advanced Radiance Facial Peel June 2009 Pentapeptides Ineffective ReVivé Peau Magnifique Madecassoside Natural/Organic Instinct Products Contain Unlisted + Mislabelled Chemicals Neutrogena Amber Bar Sculpture La Roche-Posay, Vienne, France Obagi Rosaclear May 2009 Myristyl Nicotinate Topical Myristyl Nicotinate Creams Oxofulleram Benefits of Mexoryl SX Anthelios 60 Sunscreens Anthelios SX Daily Moisturizer RevaleSkin CoffeeBerry Kit April 2009 Biomedic Facial Shield SPF 20 IS Clinical Peptides Skin Care for Dry Body Skin Site Upgrades and Updates March 2009 Safety of Micronized Zinc Sunscreens Kinetin RevaleSkin — CoffeeBerry Extract Glycation Biomedic Collagenist Powder p53 Protein Expression Photoprotective (UVB) Salen-Manganese Complex (EUK-134) Clinique Medical Skin Care (Newly Available)