Skin Care and Treatments of Melbourne Dermatology - Skinceuticals C E Ferulic Study - Methods

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Skinceuticals C E Ferulic Study - Methods

15% L-ascorbic acid and 1% dl-a-tocopherol (C+E, Skinceuticals, Garland, TX); 15% L-ascorbic acid, 10% dl-a-tocopherol and 0.50% trans ferulic acid in an aqueous vehicle; and 0.50% trans ferulic acid alone were tested.

Chemical stability was determined by high pressure liquid chromatography after heating at 45°C for one month.

To test photoprotection, each formulation, (500 ul) was applied daily for 4 days to a 7.5x square cm patch of skin.

On day 4, solar-simulated ultraviolet radiation (UVR), filtered through a WG295 Schott selective UVB band-high pass filter to filter wavelengths less than 295 nm was administered in triplicate to treated and untreated control skin, 2X —l0X MED at 2XMED intervals (IX MED = 40 mJ/cm2).

Twenty-four hours later, areas were photographed with polarizing filters and enlarged photographs were measured by colorimeter to determine erythema.

Skin was biopsied and sunburn cells were enumerated in sections of hematoxylin and eosin- stained skin.

When photodamage is extensive, it is difficult to precisely identify a sunburn cell iIn the presence of epidermal necrosis.

For analysis, whenever sunburn cells could not be accurately identified, an upper limit of 35 sunburn cells/mm was used.

Results are expressed as mean +/- standard deviation (n = 6).

The P values were calculated by Student t test.

Thymine dimers were detected in formalin-fixed skin by reaction with anti thymine dimer antibody.

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Idebenone — Prevage and Priori vs Skinceuticals C E Ferulic : A Topical Antioxidant Solution Containing Vitamin C, Vitamin E, and Ferulic Acid Prevents Ultraviolet Radiation-Induced Caspase-3 Induction in Skin : Antioxidant Potential of Ferulic Acid : Skinceuticals Antioxidant Protection - Serums Comparison Graph : Skinceuticals C E Ferulic Published Scientific Data : Skinceuticals C E Ferulic Study - Conclusions : Skinceuticals C E Ferulic Study - Discussion : Skinceuticals C E Ferulic Study - Introduction : Skinceuticals C E Ferulic Study - Methods : Skinceuticals C E Ferulic Study - Reference List : Skinceuticals C E Ferulic Study - Results : Skinceuticals C E Ferulic Video : Skinceuticals Sunburn Cell Reduction - Serums Comparison Chart :


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